This course provides an overview of the CRISPR-Cas landscape, beginning with the evolutionary origins and molecular logic of prokaryotic immunity. We will explore the expansion of the CRISPR toolset beyond simple double-strand breaks, including base editing, prime editing, and epigenetic modulation. Participants will evaluate delivery strategies for mammalian systems -ranging from viral vectors to lipid nanoparticles- and conclude with practical frameworks for experimental design.
Then, we will examine the logic of pooled CRISPR screens as a tool for dissecting complex biological pathways and identifying novel therapeutic targets. The course covers the entire experimental pipeline: from the construction of gRNA libraries to the practical execution of selective pressure in cell populations and data analysis. Significant focus is placed the diverse applications of screening in identifying molecular target for drug discovery and modeling diseases.
- Docente: AvalleLidia